An experimental protocol used to determine cellular pathways, such as precursor-product relationships. A sample (organism, cell or cellular organelle), is exposed for a relatively brief time to a radioactively labelled molecule, the pulse. It is then replaced with an excess of the unlabelled molecule, the chase (cold chase). The sample is then examined at various later times to determine the fate of radioactivity incorporated during the pulse.

Dictionary of molecular biology. 2004.

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  • pulse-chase — chās adj involving the exposure of cells to a substrate bearing a radioactive label for a predetermined time followed by exposure to a high concentration of unlabeled substrate in order to stop uptake of the labeled substrate and follow its metab …   Medical dictionary

  • pulse-chase — pulse chase. См. вытеснение метки. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Pulse-chase — Synthèse des protéines Procédé général La synthèse des protéines est le processus par lequel une cellule assemble une chaîne protéique en combinant des acides aminés isolés présents dans son cytoplasme, guidé par l information contenue dans l ADN …   Wikipédia en Français

  • Pulse-chase analysis — In biochemistry and molecular biology, a pulse chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a labeled compound (pulse) and then to the same compound in an unlabeled form… …   Wikipedia

  • pulse-chase analysis — a method for examining a cellular process occurring over time: organisms, cells, or organelles are briefly exposed to a radioactive compound (pulse) and washed; then they are exposed to the same compound, but in a nonradioactive form, for varying …   Medical dictionary

  • Analyse pulse-chase — En biochimie et en biologie moléculaire, une analyse pulse chase est une méthode d étude d un processus cellulaire survenant au fil du temps en incubant successivement les cellules dans un milieu de culture contenant un précurseur marqué (pulse) …   Wikipédia en Français

  • Pulse — Cette page d’homonymie répertorie les différents sujets et articles partageant un même nom. Pulse signifie « pouls » ou « pulsation » en anglais. Sommaire 1 Astronomie 2 …   Wikipédia en Français

  • Chase Paymentech — Type Public Industry Merchant services Founded 1985 Headquarters Dallas, Texas Products Payment Processing Services …   Wikipedia

  • вытеснение метки — pulse chase вытеснение метки. Метод, при котором клетки инкубируют в течение короткого времени с радиоактивно меченными предшественниками макромолекул и анализируют распределение меток во время последующей длительной инкубации клеток с избытком… …   Молекулярная биология и генетика. Толковый словарь.

  • Immortal DNA strand hypothesis — The immortal DNA strand hypothesis was proposed in 1975 by John Cairns as a mechanism for adult stem cells to minimize mutations in their genomes.Cairns, J. 1975. Mutation selection and the natural history of cancer. Nature (London) 255: 197… …   Wikipedia

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